Line1 orf2 antibody
NettetGeneral description. LINE-1 retrotransposable element ORF2 protein (UniProt: O00370; also known as EC:2.7.7.49, ORF2p endonuclease, ORF2p) is encoded by the Orf2p …
Line1 orf2 antibody
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NettetNational Center for Biotechnology Information NettetLINE-1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells Biotechnol J. 2024 May 3;e2000620. doi: 10.1002 ... (ORF1 and ORF2) of LINE-1. Genomic PCR analysis detected the transgene sequence in almost all tested clones.
Nettet8. jan. 2024 · LINE-1 is the only remaining active transposon in humans, and it expands by copying and pasting itself to new locations via a process called 'retrotransposition'. To do so, it is first transcribed into RNA – the molecules that help to make proteins – and then converted back into identical DNA sequences. NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11. US ES. Aplicaciones Productos Servicios Asistencia técnica. Small Pack Size Antibodies; MABE1987; All Photos (1) …
Nettetwww.ncbi.nlm.nih.gov NettetContact Cusabio to Customize LINE-1 retrotransposable element ORF2 Antibody, which reacts with Homo sapiens (Human) and will validated for ELISA, WB (ensure identification of antigen). Call: 301-363-4651
NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons. Limitations
NettetAll lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution Lane 1 : induced bacterially expressed human ORF2 Lane 2 : … everist companyNettetAnti-LINE-1 ORF1p Antibody, clone 4H1 Detect LINE-1 retrotransposable element ORF1 protein using this mouse monoclonal Anti-LINE-1 ORF1p Anibody, clone 4H1, Cat. No. … brown color bathroom ideasNettet9. jun. 2013 · The L1 expresses a 6-kb bicistronic RNA that encodes the 40 kDa Open Reading Frame-1 RNA-binding protein (ORF1p) of essential but uncertain function, and a 150 kDa ORF2 protein with endonuclease and reverse transcriptase (RT) activities ( … everist conditionerNettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein and is tested for use in ELISA, Inhibition Assay, and Western Blotting. - Find MSDS or SDS, a COA, data sheets and more information. brown color code plane crazyNettet1. aug. 1998 · Heterologous expression of human LINE-1 ORF2 in yeast yielded a single polypeptide (M r 145 000) which reacted with specific antibodies and co-purified with a reverse transcriptase activity not present in the host cells. Various deletion derivatives of the ORF2 polypeptide were also synthesized. everist body washNettetImmunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934] - Staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Product Details Summary Order Details Loading... 100% Guarantee on every product Datasheet Reviews & Publications Protocols & FAQs … brown color block shower curtainNettetLINE-1 (L1) is a class of autonomous mobile genetic elements that form somatic mosaicisms in various tissues of the organism. The activity of L1 retrotransposons is strictly controlled by many factors in somatic and germ cells at all stages of ontogenesis. everist materials colorado